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Published on Feb 19, 2014
Table of Contents: 00:09 Lecture 3.9: Amperometric Sensors - Beating the diffusion limit by Nanogap Amperometry 01:08 Outline 01:47 Glucose detection by amperometry 03:03 Essence of Amperometric Detection 03:46 Oxidation-Reaction with Diffusion 06:27 Beating diffusion limit by Redox Cycling 10:54 Beating diffusion limit by Redox Cycling 11:57 Balance the Flux, find the densities 14:10 Flux recycling for Planar Electrodes: Power of nanoscale detection 15:26 Flux recycling: arbitrary electrode geometry 16:46 A general formula for flux recycling 18:04 Finite gap Amperometry: Examples 19:24 Finite gap Amperometry: Examples 20:09 DNA Detection by Amperometry 21:57 Amperometric DNA Detection 23:04 DNA detection by nanogap amperometry 24:43 DNA detection by nanogap amperometry 25:27 DNA sequencing by nanogap amperometry 26:28 Conclusions
This course provides an in-depth analysis of the origin of the extra-ordinary sensitivity, fundamental limits, and operating principles of modern nanobiosensors. The primary focus is the physics of biomolecule detection in terms of three elementary concepts: response time, sensitivity, and selectivity. And, it potentiometric, amperometric, and cantilever-based mass sensors to illustrate the application of these concepts to specific sensor technologies.