 are tiny microscopic organisms that are actually the type of fungus. Yeasts play an important role in food and beverage industry, especially in products such as bread, beer and wine. So, in order to grow yeast in the lab, we have to provide the medium for its growth. The media that is used for the growth of yeast is yeast extract peptone dextrose. Typically, it is known as YEPD media. It contains all of the nutrients that are used for the growth of yeast. It contains peptone that serves as a source of amino acid residue. It contains yeast extract that contains all of the nutrients that are required for the growth of yeast. So, in this experiment, we will demonstrate the preparation of yeast extract peptone dextrose for the growth of yeast. In this experiment, for the preparation of yeast extract peptone dextrose, we will add 2 g of dextrose, 2 g of peptone and 1 g of yeast extract. For the next step, we need an analytical weighing balance. Place the weigh box onto the balance pan to zero out the weigh box. The lab scoop to add 2 g of dextrose at this mixture comes from the autoclave. It will help the media into the bucket. Put the bucket into the 121 degree Celsius pressure 15 psi and the time is 15 minutes. This is the pressure gauge. Press the start button to run the autoclave. At 121 degree Celsius for 15 minutes at 15 psi. After completing the cycle, use the heat resistant gloves to slowly open the lid and remove the flask from the autoclave for the growth of yeast.