 This study presents a novel localized surface plasmon resonance, LSPR, system based on the coupling of gold nano mushrooms, ONMs, and gold nanoparticles, ONPs. By optimizing the ONP size, surface chemistry, and concentration, the researchers were able to achieve a significant plasmonic resonance shift of 31 nanometers. Additionally, they were able to package the LSPR substrate into a microfluidic cell integrated with a CRISPR-Cas-13a RNA detection assay for the detection of SARS-CoV-2 RNA targets. When activated by the target, the ONPs are cleaved from linker probes and randomly deposited on the ONM substrate, resulting in a large redshift. This LSPR chip using ONP as an indicator offers a new opportunity to achieve the next generation of multiplexing and sensitive molecular diagnostic systems. This article was authored by Jacob Waikus, Yu Chang, Li Lu, and others.