 This study investigated the effects of drought on microRNA, manna, expression in the legume species Medicago troncacella. Using high throughput sequencing, 22 mannas were found to be upregulated and 10 mannas were found to be downregulated under drought conditions. Additionally, 238 putative novel mannas were identified, as well as 14 previously unknown mannas belonging to four different manna families. Furthermore, the targets of these mannas were found to be involved in various biological processes such as development, transcription, protein degradation, detoxification, and nutrient status. This study provides valuable insight into the mechanisms underlying drought tolerance in this legume species. This article was authored by Jaomingui, Chen Lei, Wang Chinsua, and others.