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What is CHT™ Ceramic Hydroxyapatite Resin?

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Published on Oct 1, 2012

For more information, visit http://www.bio-rad.com/yt/1/CHT-media
CHT Ceramic Hydroxyapatite Resin is a versatile chromatography support used for the separation of biological molecules including polyclonal and monoclonal antibodies, antibody fragments, enzymes, nucleic acids, and membrane proteins. CHT Resin is a spherical, macroporous form of hydroxyapatite. It is a mixed-mode support with functional groups consisting of pairs of positively charged crystal calcium ions, or C-sites, and clusters of six negatively charged oxygen atoms associated with triplets of crystal phosphates, or P-sites.

Bio-Rad's CHT Resins are innovative mixed-mode media that offer unique separation properties and unparalleled selectivity and resolution for a variety of molecules. These media can be used at any stage in a process from initial capture to final polishing. Two types of CHT Resins are available in three particle sizes (20, 40, and 80 µm):

• Type I has a higher protein binding capacity and better capacity for acidic proteins
• Type II has a lower protein binding capacity but offers better resolution of nucleic acids and certain proteins; it also has a very low affinity for albumin and is particularly well-suited for the purification of many species and classes of immunoglobulins

Bio-Rad's Process Separations division offers a full range of chromatography solutions for process-scale biomolecule purifications. Visit us on the Web to browse our wide selection of columns, hardware, media, and accessories.

http://www.bio-rad.com/evportal/desti...

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