 WHO urged for an extension of screening and testing due to the widespread presence of the coronavirus SARS-CoV-2. As such, a rapid and reliable diagnostic method is required. We developed a reverse transcription loop-mediated isothermal amplification, a T-lamp assay to detect SARS-CoV-2 in 30 minutes. For sets of primers were designed, targeting the viral INA of SARS-CoV-2 in the regions of ORF1AB, S-gene, and N-gene. A colorometric change indicated the presence of viral INA, allowing for easy visualization of results. The assay was tested on 16 clinical samples, with 8 positive and 8 negative results. The test results were consistent with those obtained using real-time quantitative polymerase chain reaction, RTQPCR. Additionally, we demonstrated that the RT-lamp assay could be performed without INA extraction, making it suitable for use in remote locations. This rapid, reliable, and cost-effective RT-lamp assay provides a promising solution for. This article was authored by Wei Yi Huang, Boon Lim, Kierchen Hsu, and others. We are article.tv, links in the description below.