 An effective adsorbent was synthesized from nanographene oxide for the removal of the alprazolam drug from the water sample solution. A dispersive solid phase extraction method was used with pyridilamine grafted nanographene oxide to extract and analyze small amounts of alprazolam in biological materials. Before beginning the experimental analysis process, it is important to use a simple and accessible sample preparation approach. In this study, a technique for pre-concentrating and measuring trace quantities of alprazolam in aquia samples was introduced. Analytical parameters such as the concentration factor, the limit of detection of the technique and relative standard deviation, percent, were achieved at 20g, l, 8.0g, l and 2.4%, respectively. This article was authored by Morteza-Parsaii-Arvind, Alimogemi, and Milod apniki.