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Published on Jun 26, 2012
For more info, visit http://www.bio-rad.com/yt/6/PDQuest. This tutorial explains how to optimize the detection of protein spots on 2-D gels using Bio-Rad's PDQuest 2-D analysis software. Topics covered include the selection of parameters to guide the spot analysis, such as spot shape and intensity, the representation of particular proteins on individual gels, and spot selection criteria for further analysis. Processing options such as gel warping and speckle removal are also covered.
Learn how to adjust detection sensitivity, size scale, and minimum peak values independently to tune the dynamic range of detection with immediate visual confirmation. The principles of background subtraction and streak removal are demonstrated and instructions are given for saving parameter sets for reuse with gels run under the same experimental conditions with similar amounts of protein.
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