 Microalgae is one of the most important feedstock for biofuels because of its fast growth rate, easy cultivation and wide adaptability. However, microalgae biofuels could not make an impact on the field market yet due to high producing costs. As well as the important issues, initial cell density has been studied in microalgae cultivation for many years. However, the mechanical mechanism of nipid biosynthesis up on inoculum sites has not been fully exploited yet. To analyze the mechanical relevance between nipid production and inoculum sites, we studied the effects of inoculum sites or cell growth, nipid acclimation, and the metabolism alternations in our green microalgae. In our experimental range of inoculum sites, the productivity and the safety number of FAME increased with increased initial cell density. A metabolical heat map analysis was employed to provide an overview of the metabolical profiles. Furthermore, our type correlation between metabolism and nipid acclimation in the solo canal was verified by PRS. And the 50 identified metabolics were selected for further investigations. Most of those metabolics were involved in the fixed carbon metabolism in photosynthesis. However, most of the levels of these metabolics were remarkably reduced in their highest inoculum site culture. One possible explanation for this interesting observation is that they are acceptable to microalgae cells in culture. We therefore asked whether high cell density netted the light permeability into the culture and led to less illumination to cells, which then affected the oil productivity and the quality. To evaluate this hypothesis, we changed the light intensity to improve the growth and FAME of the culture with the highest inoculum site. The draft of the illumination essay revealed that increasing light intensity could improve the PUFA composition and nipid acclimation of the solo canal. Based on this study, we propose it necessary to consider the light intensity as a major factor for FAME quality during scanning culture in the field for biofuels production. Thank you for your attention. If you are interested in this study, please list about technology and bioengineering for more details.