 This study used RAPD and SSR loci to investigate genetic relationships in a group of 22 raspberry accessions, finding high genetic variability and clearly delineating genetic relationships through cluster analysis. The highest genetic similarity was found between two polish cultivars, while jewel varied the most from all accessions. RAPD and SSR markers were confirmed useful for discriminating among closely related raspberries and determining genetic variability among cultivars, which may aid breeders in planning their breeding strategy. This article was authored by Magdalena Simla, a god attack, Adam Kula, and others.