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P. falciparum high-throughput Luciferase assay

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Published on Aug 29, 2009

Malaria 96 well high-throughput Luciferase assay.
Author: Flor Caro
DeRisi Lab UCSF
Malaria parasites transfected with a renilla luciferase reporter construct are released from the red blood cells (RBCs) by saponin lysis. Excess hemoglobin is washed by vacuum filtration on a 96 wel PVDF filter plate while parasites are retained. Parasites are lysed using the renilla luciferase lysis buffer and collected by centrifugation on a white assay plate. RLUs are measured on a luminometer.

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