 Unconventional protein export slash secretion, UP slash UPS, is a process by which proteins are released from cells without the use of traditional signal sequences or vesicles. This process requires multiple mechanisms to ensure successful delivery of the protein to its destination. The first step involves the translocation of the protein across the plasma membrane, which can be achieved either independently of a vesicle or via a vesicle. Once the protein has crossed the membrane, it must then enter the lumen of a vesicle, where it will be delivered to its final destination. Finally, the vesicle must fuse with the plasma membrane and release the protein into the extracellular environment. This article was authored by Jingjianfei and Guilian.