 The study found that the sterilizing properties of an atmospheric dielectric barrier discharge plasma device can be enhanced when air or oxygen are used as the gas medium. This device generates long-lasting active species which flow through the silicone tubing and complete sterilization within 30 minutes. The main factors responsible for this sterilization process include hydrogen peroxide, HNO3, and nitrous oxide, N2O5. Additionally, the presence of keratin, aspartic acid, and epichylinic acid in the bacteria spores had no significant impact on their sterilization. This article was authored by Rionomuto and Nobuya Hayoshi.