 This study aimed to create rice-brown oil nano-emulsions using low-energy emulsification methods and evaluate their physical stability, irritation potential, and moisturizing activity on volunteers with normal and diseased skin types. The results showed that the developed nano-emulsion was stable, had a low irritation potential, and improved skin moisture and maintained normal pH values when applied to human skin during in vivo studies, indicating its potential to treat skin diseases such as atopic dermatitis and psoriasis.