 The study examined the effectiveness of various enzymes and conditions on the hydrolysis of wheat starch. The results showed that increasing the enzyme to substrate ratio, E-slash-S ratio, decreased reaction time but reached similar concentrations as lower ratios. The most effective enzymatic deactivation method was found to be pH adjustment. Additionally, glucose did not have any inhibitory effects on the enzymatic reactions. This article was authored by Juan Camillo Acosta-Pavis, Laura Alzade Blandin, and Angela Adriana Ruiz Colorado.