 In this study, spider silks, SS, collagen, and polyurethane, PU, nanofiber scaffolds were fabricated by electrospinning technique and their physical chemical properties and in vitro biocompatibility were investigated. Results showed that SS scaffolds had stronger hydrophobic surface, superior water stability, higher porosity, and promoted better cell growth and proliferation than other scaffolds. Suggesting the potential application of SS nanofibers as vascular engineering scaffolds. This article was authored by Chuanglong He, Lei Zhang, Hongxing Wang, and others.