 Dibrio perihemolyticus is a bacterium commonly found in seafood and can cause acute gastroenteritis in humans. A new detection method has been developed combining multiplex polymerase chain reaction, PCR, and a lateral flow dipstick, LFD. This combination allows for rapid and accurate identification of pathogenic V. Perihemolyticus, from seafood samples. The method was optimized and evaluated using agarose gel electrophoresis and a universal LFD strip. The results showed no nonspecific amplifications and a limit of detection of 0.78-ing DNA slash reaction. Additionally, the method was tested on artificially contaminated shrimps and successfully detected the presence of pathogenic V, perihemolyticus at concentrations as low as 10 CFU slash 10 G of sample. This method may prove useful in developing a rapid and reliable detection kit for use in the food safety sector. This article was authored by Jirikrit Seytang, Fatapong Sukapat, Surya Palami, and others. We are article.tv, links in the description below.