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Published on Dec 16, 2012
Bile solubility test is performed to differentiate Streptococcus pneumoniae (pneumococcus) from other alpha-hemolytic (viridans Streptococcoci) cocci. Addition of bile solution activates amidase enzyme resulting in induction of autolysis. The test can be performed on colonies on agar or using broth culture. While 2% bile (sodium deoxycholate) solution is used for agar test, 10% solution is used for broth test. Disappearance of colony (agar test) or clearance of turbidity (broth test) is considered positive identification of Streptococcus pneumoniae.