 we have a very interesting thing to announce now. So, the the past many years people have been working, the past many years people have been working on different areas and we have to recognize some people who are standing out, some groups which are standing out not just people ok. So, it is essentially not individuals, but the group of people. So, we have some awards as usual. So, the first one is DD Cosambi award for cultivation cultivating scientific number by remodeling the curriculum at the delivery stage. So, this essentially what you people have been doing, the students have been doing largely is being incorporated into the syllabus or how to while while delivering the curriculum in the classroom. So, some culture of you students have been sort of transferred to the teachers and through them to the larger student body and the the the education system. So, you can see 10 such groups MHSS Goa that Sucheta Naik is one of the leading persons there, IAS Jaipur Srimoyee, Cochin College Bilaspur Komal Singh, Cochin College Manju and Shreech Smita, Bliss Sapparkatti, I do not know whether I am pronouncing it right this is the first time I am hearing this place it is actually on the border of very close to the border of Burma ok in Assam ok. Sushant Singh, the the Al Singh called Bliss ok, the Al Singh college Chitralekha, Bliss Silchar, Krishendra Roy you must must be seeing Krishendra Roy's a lot of photos he is organizing on various things. Ganesh Bhasky and group and Ranji College, Adarsh Vidyalaya Shreedevi, KV Arariya, Nirmal. The second group of awards people who are getting the awards this for in the name of SK Mahajan awards for science education and research initiation. SK Mahajan I do not know I do not think we have given an award till now. SK Mahajan has been a very very interesting veteran scientist in in BARC and he passed away some some years ago. His wife Bhaktavar Mahajan has been here a faculty and she is very very keen that we should have an award on his name and we are also he has been a friend of us and he has been an inspirer for all these type of work. So in innovations in Hydra culture Moina so ARIF lab who is ARIF here is ARIF here. So we have people say that Obaid's lab, Vidita's lab type of thing people say that so this here is an ARIF lab you must have heard about it and people who are working and joining now particularly Obaid's, Harshada, Vipti, Ashwini, Sheetal who just joined recently and ARIF and Sarthak. Now Sarthak has also created some sort of an innovation in Delhi by starting a like I think you heard what ARIF's group have been doing they have been developing Hydra into a sort of every homes business is it not you know you can you can keep it in every home like Jee and said about desktop yeah type of Hydra culture. Something similar what it has not been really evolved but there is a seed of that Sarthak has sown is on Moina he is developing he and his groups are developing it into a Parkinsonian model studying its mortality and stuff like that and which he started actually in Kottayam there is an indoor university center at Kottayam where he went for his internship and those people actually has adopted that yeah Dr. Mohan Kumar who has been known to us from Kolkata he has also adopted it so the award goes to that and the third award is Anil Satgopal's People Science Awards for Population of Science. Now I think if anybody has been looking at our instant messaging systems you must be noticing Tushar Tushar's group from Konkan has been one of the one of the major contributors to popularizing science and particularly popularizing science in their area and particularly very very extremely brilliant way they have been doing it and very consistent way they have been doing it one of their contribution standing a contribution is slice of every month slice of the season every month I think that's why representative sample of the the Maharashtra Sathya Maharashtra they have been doing it which other people are emulating it from other we have several such slices that's coming from all over the country now I don't know whether we'll have time to time to screen all those things Jay will be doing that okay so that this is what I wanted to announce and it's a very happy occasion to announce this particularly in the name of Didiko Sambi, S.K. Mahajan and Anil Satgopal I think I will stop at that and be the for the Jay thank you very much okay so one of the major activity in which a lot of people have been contributing in cube since actually last year is mango mapping okay so this year this has been you know one of the one of the activity in which we have got a large scale you know participation from across the country people have been sending pictures and so many pictures that you know now you know we have so much of data within one year okay so one of the yeah yeah so one of the thing that you know what we had started is that to map the mango so initially what people were sending the pictures of the the mangoes the mangoes mango trees you know from different you know at different time in every month actually so what they what we started to make a hypothesis is that you know when do the mango tree flower so that was the major question so we have we last year when we did it we found that you know as we so first the mango tree starts flowering in the lower in the lower latitude like you know which is you know in India it is near Kerala for example yeah because yeah Kanyakumari yeah because because that is you know very near to the equator so it's actually 8 degree north latitude so it is very near to equator so it gets a lot of you know first it will get the light period maximum amount of the light you know and then the thing will the light will be migrating to the upper places so we have started to see that you know when we started to map this we have got some data here yeah so this is a map which we had made in january and february uh yeah one second i will just yeah january and february 2017 where you can see that you know the yellow ones are the flowering flowers and so and the green ones are green ones are fruiting so first thing is the you can see that fruiting is first is all around near the you know south southern part of india so it is it means that the first flowering happens there first law and then the flowering migrates there and till the until the flowering will migrate there the lower one will be started to fruiting started the fruiting so and you can see that you know as far as so what we have found that there is a very less amount of flowering in the uh what you call northern regions and as we then if we go to the next month you know then february to march we found that you know there is a lot of uh the flowering which is happening across the india the in the northern part also it shifts so there's a shift of the flower the fruiting before it was only in the southern part now it is also happening started to happen in the uh in the what you call the northern part it means that the first the early flowering happens there it begins from there and then it migrates so this was the pattern which we observed and then yeah uh so i think yeah i missed the one yeah so this was the very first so this is actually the beginning one september two january this is you know this is what you call around in the uh the winter actually when it is no starting starting winter yeah autumn ending and yeah so at that time see the flowering first begins in the karela so there is no the gray ones are the ones which are not flowering and not even fruiting so mumbai is coming there flowering doesn't even start there but it starts in the karela so this was the pattern which we observed and now this year also we have repeated the same thing and we have got the similar kind of pattern so here is this year's this is a data of this month sorry december month of the december so in december the flowering is happening there again the what you call the southern part it is coming close to the uh what you call uh the mumbai or goa this is goa in maharashtra vatnagiri side but in if you go towards this uh like bihar ararya or asam that regions are actually not having any flower and not having any fruit december december of 2017 so this will be around you know yeah so two months back so it means that it has started in the southern part and so there's a clear cut distinct you know what you call the difference which is there but you can see that two looks like looks like yeah but there are two things like you know around have not started flowering okay so that may be because we have not got some enough data from there it could be yeah it could be atypical or it could be you know that we have not got enough data if we look for around for some more data actually we have less data for that if we look that it could also be you know some some of them could be started to flower but definitely it is not started in the upper part like you know the the bihar asam you know up kind of places so this is a pattern which we have observed now what is this actually why we are doing this okay first thing is of course that you know you get a large scale part of the people are becoming sensitive to the to the you know environment around them they are taking pictures anybody can do it even a you know school student can just click a picture and send it to us you know we have so many groups he can if he doesn't have mobile he can take a help from parents and all but the thing is that that is one of the thing but our question is more than that what we are understanding is that you know we want to under we want to look at you know there's a why this change is happening why actually this is happening this is because actually because of it's called a photopedidism there's a the light is affecting the flowering so tree there's a tree in kerala there's a mango tree in mumbai there's a mango tree in you know bihar but all of these are these trees are could be same but these things these trees you know are having different behavior they are flowering at a different period of time that is only because we are saying that it is a it's a direct environmental effect on the plant you know and the environment effect means it's a light effect effect of the light so there's a photo period the how much time period the how much light period will the plant get it depends on that when it will flower so it so it has to go into if you go into deeper than it has to deal with the you know how there are genes which are responsible for flower so that means that at that period of time so so once you get that much of light period that much of accumulation of the you know light period then it will trigger some of the genes until that until that you know until that time it will not be activated that gene will not be activated so there are many genes so you can go up to the level of you know how does the flowering what is the basis for flowering or what is the you know biological you know mechanisms responsible for this flowering changes so this is one of the things so we have got a lot of I will just show you some of them yeah so so this is okay so these are some of the things which we have got from this is from Supriya and Orissa this is coming from Orissa Kattak and then they have actually you know they have been looking at when does the flowering happens so no flowers are budding leaves observed this is coming from so this is Orissa you know yeah Kattak so no flowering until till 15 December you know then there is again new leaves are coming new sprout of leaves are coming but there's no mention of flowering so okay there's new leaves are there but there's no so again there's a question that that before the before the flowering happens just new leaves are sprouting so why that you know yeah the our question was that you know is it that because their flowering flowering is postponed because there is a leaf which is sprouting so there is a vegetative growth but there is no what you call the reproductive you know the growth is it because there is a sprouting happening because of that vegetative growth the reproductive growth is getting prolonged that is one of the question which we are thinking so so in okay inferences I think yeah majority of the mango trees of this chosen area of observations sprout new leaves before flowering so this is the conclusion they are they're drawing that before the flowering the new leaves are sprouting so whether there is a vegetative versus reproductive kind of you know aspect to it then okay then I will move to other things that we have got a lot of data from as Arunan sir mentioned that we have got a lot of data from Konkan this is again the slice of the season that there's a lot of seasons a lot of our biodiversity or you can say the animals which are there around whether how the our environment is affecting them means how what kind of animals we are finding in different seasons so this is just a collection I'm not saying that we are we are not looking at we have not looked at the pattern emerging from it but we have definitely got a lot of contribution that there are so many butterflies we have got from so this is a person Tushar who has been sending all these entries so every month so this was the month of December then we have he has also sent in November you know caterpillar he has all these collections from is around the surrounding and around his school area so this is a contribution of January which Arunan sir only talked about then I will yeah this is I think again from October month so and we have one contributor from Delhi who has actually documented you know recorded all the this the butterflies in his camp that is ANDC campus so he he shows some around there are some 15 species he has recorded he himself alone but there is an interesting thing there so these are around 15 species you know I don't know the yeah names are some you know pale grass blue butterfly Tridex yeah this is by Subram this is by Subram so Tridex patch or there is another one which is Dennis so there are many so he has actually documented 15 but the interesting thing is not that you know you are getting of course you will get the butterfly but our question is that what you know how many different kind of butterfly you will get it yeah so how many different kind we have got one paper one recent article which says that I don't know 50 50 species of butterflies but that is very less there cannot be a 50 species of butterfly we don't expect because there's so much of you know for example you know gardens around you know so many things but house this is an urban area as I'm talking not the not the jungle or things forest like but urban areas 50 is documented which means actually whether the butterfly species are decreasing numbers of the species are decreasing because of the urbanization we don't know yeah so this is very in that way it is it is a interesting thing to know okay then we shall be yeah we shall be moving to yeah one more thing okay so this is about the what I just mentioned about the flowering seasons and the different kind of biodiversity around us but I have another thing which is on the what you call the the drosophila so you have every we have discussed so many things about drosophila circadian students discussed about the circadian rhythm things and then they also discussed about the evolution aspects in the drosophila but we are also looking at we have just now started what you call introduced just introduce I'm just introducing the new system new drosophila can be used for as a model system for immunology studies we have just introduced this so I'm just for so there is a lot of so drosophila has actually you know noble price now recently noble prices have gone for the immunology studies so one of the way in which what so one of the studies by by the these three scientists so you know who have got noble prices uh Hoffman you know and he has got noble price in 2011 and the what actually they found out is that is that you know how the how the body senses the pathogen so initially so what actually there is a role of innate immunity you know there will be macrophages all these things we know but what actually what what receptors will be uh you know detecting that there is a pathogen so because there are so many pathogens around okay there are plenty of different types of pathogens around so there cannot be a one receptor or kind of things so all these things are questions which are there but they have found out the receptors which are involved in this kind of first so there's a first line of defense the receptors they have found they are called as told like receptors so they're involved in the innate immunity means so they actually are present so Hoffman and uh his colleagues made his pioneering discovery in 1996 when he's uh investigated food flies combating infections now this can be easily done in any simple lab like even cube labs are you know it can be done so they have access to flies with mutations in tall genes told like receptors genes when Hoffman infected toll mutant flies with bacteria so they are infecting the flies with the bacteria or a fungi it means that they're growing them with the bacteria yeah in the medium bottle he discovered that toll mutants infected with fungi died because they could not mount an effective in fact uh effective immune response so which means that if that if that receptor is blocked or mutated the organism the fruit fly will not be able to detect the pathogen and if it is not detecting the whole lot of you know immune response will not be triggered it means that the immune response is coming from that receptors so that's so important for our immune whole immune system to know so that is why the it's a huge contribution so again the thing was that you know okay what how we can be it can be used to us for example we have this drosophila who has which is actually we have this larvae of the drosophila you know so larvae actually if you just put the puncture the end of the larvae the lot of fluid will be coming out and that fluid is actually the the hemolymph the the fluid has the lot of you know blood blood cells so that blood cells will be having what they will be having lot of you know all these things the the the lot of immune immune immune cells so you can get you know myeloid lymphoid all these erythrocytes lymphocytes so we can actually observe them you know you can see the blood cells so if you for example if you infect the if you infect the animal with a bacteria these bloods the different kind of immune cells should be getting higher because the immune immune response is now triggered so number of them will be higher so you can get the higher number means the WBC count will be increased like you know you can maybe you can get the macrophages level is increased you know or B cell level is increased so all these things are actually you know responsible so those things can be easily and I said in a simple thing so yeah yeah yeah we can stain so the thing is that we the the the simplicity is that we can stain with the normal stains like you know you can use some fields differences in field stain are available leishman leishman not but field stains are there which actually stain because they are there's an acidic there's a difference in their acidity some are acidic some are basic so they can be stayed stained easily so this was the other thing so I just wanted to yeah so I think just one minute I can if I can play a video so then I can just move to the movie session oh I think it will not load yeah we have the videos here but I'm just one video you can get one minute yeah done yeah lights if light I don't know it will work but I will just check once if it is not yeah we can go to the movie screening session yeah yeah no I think it will take time yeah leave it leave it okay so I think it is not going to yeah these are just hemocytes and just moving some random this is video but okay yeah but yeah I will just move to the next movie screening session so yeah so everyone has got the small sheet of paper has everyone got it is anyone there who has not got the paper okay so we will be so we will be now showing some movies and that movies yeah grade star yeah five star is maximum we have the names for the different places can names are there okay maybe you can write down the names that is good because yeah yeah yeah yeah but star means you know five star if suppose there are three movies who are having three stars or three movies having five stars okay so you can just write down the names accordingly so I will play in this order only so no problem so maybe you can write first is butterflies yeah otherwise you can okay yeah just butterflies from kochi okay if there is any problem in the name also again from butterfly only so yeah you can write them so now I'm going to next video this is of something on butterflies but this is it is coming from goa next okay this is actually some some seasons or something like that but this is a very important video I'm just you can I don't know you can mark or not but whether it fits in the criteria of marking but I'm just saying that it is a important because it is a lab you know he has made this the person is called his name is Nirmal he started a lab cube lab in the area and this is a video of just that I don't know I don't know it I think it has no volume yeah the one who is watching through microscope is the person so he has started a lab in no it has no volume okay this is mango tree spinology this is mango flowering so these are the flowers of my name Nirmal the person's name is Nirmal this is for me that is for you this is for me so I can only see this yeah there is a yeah this is one video also okay yeah okay yeah we'll move to the another group so yeah so another group is this is from Guwahati this is a bliss group from cube group from Guwahati so I will just play this this is about the DNA extraction this is a group which I had also presented the the DNA extraction he's going to present they have also sent the in the last session but they have refined some of the things now and they have sent back again the requirements are clear glass and beakers common salt because banana glass rock spatula needle distilled water shampoo teenage rubbing alcohol that should be ice pool and a ziploc plastic bag now how to do the extraction first we have to remove the peel of the banana after removing the peel we have to put it inside the ziploc plastic bag and then we have to seal it and now we have to smash the banana we are doing the smashing because this will break the plant matter now we have to take a clear glass and we have to add two teaspoon of common salt using this spatula this is first spoon this is the second teaspoon now after adding the salt we have to take the distilled water now we have to pour the distilled water half cup it's almost the half cup now we have to steer the solution very gently now the solution we have is the salt solution now we will add two ml of shampoo that we use in the house now that's two ml of shampoo now we have to stir it well and we have to make sure that no bubbles should be there the salt we had added will later help the DNA to stick together now we have to add this solution to the ziploc plastic bag of squished fruit we have to add enough so that we have a nice mixture that we can see through it that's probably about one third of a cup of salt solution now now flatten out the plastic bag to remove most of the air and then seal it up gently squish the liquid around let the mixture sit for 10 to 20 minutes to give the detergent time to release a lot of DNA after 20 minutes so the next step is to filter the banana smash so we will do it with the help of peanut we will place the peanut on a beaker and then we are going to pour this whole banana smash into it now we will gently squeeze the banana smash to get a liquid now we will place the peanut aside and we will take rubbing alcohol which is ice cold so we will add the alcohol as much amount that the liquid is present here so we will left it for three to four minutes so as you can see there are two layers the layer at the upper surface is actually the alcohol in which the DNA is mixed and the layer which is settled at the bottom is the juice of the banana mash we have our DNA extracted and it is submerged in the distilled water so now we are going to add this in our DPS solution here we have taken 10 8 ml of glyceric acid with 0.2 ml of H2SO4 and 170 of DPS DPS powder we are adding the DNA here after it we will place this test tube in the water bath it is on 100 degrees Celsius that means the water is boiling here so we are going to add this here don't gently place it and we will close it and we will wait for 15 minutes 15 minutes we are going to see what it had happened it's too hot yes we had DNA the DPS we had added has turned into blue color dyed blue so hence proved that the DNA we had was the DNA thank you yeah last time they didn't have the control so now they have they are showing the controls okay okay okay this is again the another one from for the same DNA extraction hello everyone today we are going to extract DNA in a very first let us introduce ourselves hi and veneetha hi i'm kriya and our friend who is shooting the video for us is Sunandha the materials which we are using today for our experiment you can find easily in our home before studying our experiment we would like to tell you that we took variation in our surfactant salt and dye and the variation in the surfactant are vim life point and lux and the variation the salt which we used are crude salt and common salt and the variation this time which we used in the stirring of the solution is firstly we didn't state a solution at all secondly we stirred it for two minutes and third we stirred it for five minutes after conducting a number of experiment with our variations we got to our standardized protocol which we are going to demonstrate today in front of you so let's start our experiment firstly let's have a look at the material which we will need today for our experiment our source which we are using today banana we have some water salt alcohol mortar and pestle all those 10 grams of this soap we have taken vim and a strainer so our first step will be will be smashing our banana as you can see we have chopped our banana finely so that it will be easy for us to smash it we'll smash our banana using a pestle and a mortar smashing the banana helps us in breaking apart the clusters of cells from each other so we have smashed our banana as you can see banana is properly smashed so now we'll keep our banana smashed aside and we'll prepare our solution so for preparing our solution we'll need a cup some water we'll fill our cup almost three fourth with our water the water we'll add almost 10 round of vim you can use any liquid dish soap then at last we'll add half teaspoon minutes so guys we are back after 10 minutes so after 10 minutes we'll filter our mixture using a cup and a strainer we are using a strainer for filtration you can use any cloth like muslin cloth or cotton cloth so now you can see that the debris of the banana smash which did not get filtered are left in the strainer and the solution got strained in the cup so our last so our last step will be will make a layer of alcohol in the solution alcohol helps to precipitate our precipitate out the DNA out of the so now you can see that the debris of the banana smash has been left in the strainer and the solution got filtered through the strainer and will be will add a layer of alcohol in the solution alcohol helps to precipitate the DNA out of the solution be careful that you use chilled alcohol for precipitating the DNA so you can distinguish here the two layers of solution the down part is the solution of the banana smash and the above part is the alcohol the more we let it set the more DNA gets precipitated out we can see after five or ten minutes that the DNA which has been precipitated binds together to form a cottony mass so you can see that after five to ten minutes all the DNA which has been precipitated got bind to form the cottony mass substance in this video we'll confirm the DNA which we have extracted in our previous video so let's get started so let's have a look at the material which we are required for our test we'll require B cup test tube neither for taking out the DNA from the cup 2 ml of H2O4 then ml of glacial acetic acid 0.1 gram of DPA which is our reagent our extracted DNA and gloves as we are dealing with acids so let's get started first we'll take a test tube and we'll add 8 milligram of glacial acetic acid now in it we'll put 2.5 milligram of concentrated H2SO4 now we'll put our test tube inside it we need to submerge the whole solution in the water bath so after 15 minutes you can see that our solution has turned into deep blue and this confirms that our solution has DNA so that's all for our experiment thanks for watching us hope you like it today again it was yeah because this is also DNA extraction so I'm just okay no you don't want this okay we don't want that okay first we'll go to then maybe last last we'll go to that so see he actually has done it yeah he actually has done this at at our home so this is our home lab kind of thing which he has made so yeah we'll move to next next is what mango market okay yeah okay this is uh this is a first first mango which came uh and this is a video which person who is from from goa she has taken from the market so because the market is a you know kind of marker for mango you know when the mango comes in the market we can come to know that you know it may be when the first mango comes to the market that will be very interesting to find out in in various parts of the mango in market mango phenology next is kerala so this is a place where first the mango flowering starts she shows the flowers also this is mango phenology from uh goa yeah this is from jaipur so this is again true flies can be raised easily wherever fermentation is in progress in laboratories they are usually not fed directly with override and fermented fruits because the fruit culture media will become too soft by the time new flies begin to hatch due to the process of fermentation therefore fruit flies are fed in more solid culture media enriched with all the nutrients essential for survival and growth of life materials required for the preparation of media conical flasks spatula cotton stirrer measuring cylinder media for 250 ml involves various components dextrose 12.5 gram adds as a source of carbohydrates sucrose 6.25 gram again as a carbohydrate source mains powder 20.75 gram enriched in carbohydrate again as a major source but also includes proteins fat and some other micronutrients yeast extract 3.75 gram acts as a source of vitamins digested nucleic acids a bar powder 4.5 gram it is a solidifying agent for the media orthophosphoric acid 0.17 ml and propaneic acid 1 ml added to avoid bacterial and fungal growth these wave components are transferred into a glass and then total volume is made up to 250 ml by adding distilled water then with the help of a glass stirrer the ingredients are mixed in the flasks conical flask is then kept on the hot plate for dissolving the media components and instead continuously slight bubbling starts on the top layer the hot plate is switched off and the flask is removed we will plug the mouth of the flask with the cotton plug and later it is auto played fruit flies can be raised easy this is okay we have got only this is only one video we have got from spider so she has been working continuously on spiders and documenting a lot of spiders this is from kerala so the person's name is uh so we can find out whether all of them are spiders or something else also yeah so this is from so this is uh yeah from think lab uh delhi and this is yeah next is again from jaipur this is again just the way to transfer the flies off media overcrowding of flies in purple also the flies are transferred to make single line culture or to perform any experiment for further studies requirements a culture bottle containing flies an empty bottle containing only solidified media and no fly a sponge and east granules procedure two to three east granules are added to new media bottle in the geotropic thus most of the flies are found at bottles neck so a different and somewhat interesting procedure is applied to transfer them gently tap the flies down by softly tapping the bottle on soft surface such as sponge to reduce the amplitude of course and to avoid the breakage of bottles the flies will fall down at the bottom and will remain there for a few seconds take off the cotton plug from the bottle and place another bottle containing fresh media in inverted position such that the mouth of both the bottles join each other this joining should be covered with a hand the whole setup is rotated anti-clockwise and tapped on the sponge this tapping provides job due to which flies move in new media bottle different stages of larva and pupa the culture bottle is placed in upright position after this place the cotton plug on both the bottles new culture bottle is labelled accordingly precautions cotton plug should be tight enough to avoid escape of flies culture bottle must be handled carefully the job applied to the flies must be to transfer flies there are several reasons this is on trapping of the fruit flies which actually many cubists are doing let's learn how to trap fruit flies we will take a transparent bottle for trapping fruit flies bottle should be clean and dry and liable properly here we are mentioning location and dates on a bottle now transfer the banana fruit pea in it to a trap flies clean the mouth of the bottle using cotton keep the trap near the spin or any area where there are possibilities of trapping fruit flies check the trap in proper time interval as we can see now flies are there in trap we'll close the bottle with cap make some holes over it to maintain proper elevation we can transfer these flies in media bottle let's learn how to trap fruit flies so i think it is done but only one is remaining yeah it's okay okay uh the kanpara uh dna extraction just a little bit of yeah this is Ani Kohli along with my classmates Nehna Swajjali and Vishal Nishtar of class 11 from the school can be with their electronapola will be conducting the experiment of isolation of DNA from tomatoes using a simple method materials required for these experiment are tomatoes, plastic bag, salt, detergent, glass of water, muslin cloth, ethyl alcohol and empty beaker and glass walls first we will take the crop tomatoes and put it inside the plastic bag now only crush the tomatoes after crushing the tomatoes keep it aside and take 80 ml of water in a beaker or glass and add 2-3 spoons of detergent to it the detergent will break the cell wall and help the DNA come outside now mix it well with the water make sure no bubbles are formed small quantity of salt and dissolve it into the solution the salt will help to bind the DNA together at the solution slowly inside the plastic bag make sure that the air is removed from the plastic bag now mix it plastic bag aside in normal room temperature for about 20 minutes beaker and cover the mouth of the beaker with muslin cloth filter the pulp present inside the plastic bag to the empty beaker equal to the level of solution in the beaker after filtration make sure that the alcohol is chilled now we will wait for some time till the DNA appears you can observe some white cottony substance in the solution this is the isolated DNA of the tomatoes now using a needle swirl it inside the beaker and take the DNA fibers we will collect it inside and at 10 dots so this is the extracted DNA of tomatoes after we take extracted DNA and mix it with the beaker reagent and clay cell acidic acid we boil it for 15 minutes in hot water bath after 15 minutes of boiling you can observe that the color has changed into blue which confirms the presence of DNA hope you liked our video okay so this the where in which they show the how it is different from see you can see that how they are doing they are they are actually taking some you know with things which are available in kitchen and they are extracting the DNA and showing so but the how it is different from the classroom practical you know because we always use these chemicals you know like SDS or detergent so instead of SDS they are using some another detergent and they are doing so this is the way in which you know they are showing how simple it is to do science so I think that is what we are able to you know from such a brilliant contribution from across the country so I think you will be you want to say add something before we close the session yeah okay yeah so we have okay yeah but we have collected so the we have you have marked this yeah pay on the paper the movie so we'll be analyzing using this in order to okay so I think the the type of innovation I think we can claim this time was that we were constrained to have a Bombay meeting for QB winter but then we expanded it to the whole country and people have been watching even now I think we were getting feedback from them because we have this streaming that's one thing which along with that we have this contribution that's coming from people as movie makers club so we have movie makers club all over the country now and also we had this season slice of season stuff so both these things gave us enough participation today and so it is no more a QB winter Mumbai meeting it's an all India meeting though we didn't have to foot the bill for their travel their travel and stay and stuff like that we had those constraints this time that's why we could not but then we transcended that and thanks to the technology thanks to these people who are helping us and thanks to all the participants who are very very very much involved in this and thanks to you people who came here now I can see somewhere around one fifth of the people are here but I think thanks thanks a lot so if I think please make some comments in the continuously stress type of people you know you have been doing the reasonably well but you have not been contributing regularly so that you will not get the feedback you are not getting the feedback so it's a it's a wonderful thing that we have a huge sort of audience if you look at the the instant messaging system you will see that people have been watching and appreciating things okay and that there was a correction from Delhi coming saying that the one of the movies was what Jay Jay said that it is it's from Goa it's not actually it's from the Alson College Delhi so they were correcting us while watching this so it was a wonderful thing that so many people have been involved in that across the country okay thank you very much so carry on okay thanks a lot for all people who have been from across the country watching and participating in the whole process yes wave to the camera where's the camera okay thanks thanks a lot yeah