 The study aimed to identify and characterize multidrug-resistant strains of Staphylococcus aureus among healthcare workers in Pakistan. The results showed that 80% of the isolates were methicillin-resistant Staphylococcus aureus, MRSA, and 45% were multidrug-resistant. Additionally, 36% of the MRSA isolates carried the methicillin, resistance mecha gene and 31% had the pantin-valentine lecosidin, PVL, gene. This article was authored by Maria Rookin, Humaira Jamil, Habib Ali Bakhari, and others.