 The study aimed to investigate the potential protective role of alphalipoic acid, ALA, on methotrexate-induced lung injury, mealy, in rats by measuring various parameters such as interleukin-1, beta, malendialdehyde, glutathion, tumor necrosis factor alpha, myeloperoxidase, and sodium-potassium adenosine triphosphatase. The results showed that oxidative damage of MTX in rat lung was partially reduced when combined with ALA, as evidenced by lower tissue malendialdehyde levels and higher tissue glutathion and Na+, K+, ATPase activities. Additionally, histopathological examination revealed that lung injury was less severe in the MTX group compared to the MTX+, ALA group. This article was authored by Hussain R.Pag, Mehmet Gol, Yusuf Adema, and others.