 Today I'm going to be talking about aseptic technique, which is used to maintain sterility when you're working with things such as cell culture to prevent contamination of your samples. So this is done in a laminar flow hood. You move the front of the hood up. There's usually some marks or indications how high this needs to go to maintain proper air flow inside the hood. Another important thing is to always wear gloves and a lab coat so that everything going into the hood is sterile. Before working, you can spray the hood off with 70% ethanol and I'll see your gloves as well and this will help further clean anything inside of the hood. Then everything that's going inside of the hood, you can spray off with 70% ethanol as well. So I'm going to demonstrate how to properly transport media sterile. So it's important that you loosen all the lids of the media bottles or tubes that you're working with and this will help prevent you from touching things that should not be touched when you're trying to force open a bottle. Second, opening the pipette, you want to make sure that you're not touching anything with your hands. So everything that inside of the package is clean and you want to maintain that. So open it carefully so that you're not touching anything and then you can take your pipette and discard the wrapper for later. Now that the media is open, use your one hand to take off the cap of the media and use the other to draw out the media. When you're finished, you can put the cap back on. It's important not to pass over the top of the media bottle or any open container without the lid on because this can cause contamination. And with the other hand, you can take the cap off this media bottle or this tube and transport your media. When you're done, put your lid back on and if you're done with your pipette, you can put it away and discard it for later use. You're discarded.