 The spark discharge method, SDM, was used to synthesize copper and copper oxide nanoparticles, NPs, on cotton fabrics for antibacterial textiles, and various analytical techniques were applied for characterization. X-ray powder diffraction, scanning electron microscope, and dynamic light scattering apparatus were used to investigate the structural, morphological, and particle-size properties of the synthesized CUO-NPs on cotton fabrics. The results showed that a concentration of 100 ppm is sufficient for killing 106 CFU, ML of bacteria, and the synthesized CUO-NPs are highly effective against both Staphylococcus aureus and Escherichia coli. The antibacterial fastness properties were also tested after 15 cycles of washing, and the results confirmed that the synthesized CUO-NPs maintain their antibacterial efficiency. Therefore, SDM for in-situ synthesis of nano-CUO on cotton fabrics in batch systems is a promising method for producing antibacterial textiles. This article was offered by Sheila Shahidi, Amir Jamali, Sonos Deilal Sharifi, and others. We are article.tv, links in the description below.