 xylonease and xylonease enzymes can considerably enhance hydrolysis effectiveness of cellulase cocktails when added to pretreated lignocellulosic substrates. The blocking effect of xylone was one of the major mechanisms that limited the accessibility of the cellulase enzymes to the cellulose. However, the synergistic interaction of the xylone enzyme and cellulase enzyme enzymes was also shown to significantly improve cellulose accessibility. This article was authored by Sadler Jacken, Errant Spalder, and Hu Jinguang.