 The study reports the green synthesis of silver nanoparticles from a known Esquimosa leaf extract in 20 minutes using high-throughput characterization techniques such as UVVIS spectroscopy, FTR, TM, FESEM, XRD, and Zeta potential measurements. The phenolic compounds present in the aqueous leaves extract facilitate the reduction of silver to nanosilver. Electron microscopic studies reveal an average size of 52 nanometers for the nanoparticles, which are highly stable with a Zeta potential value of minus 33.6 mV. The study also compares the antibacterial efficacy of silver nanoparticles against Escherichia coli using disk diffusion and microtiter broth dilution methods, suggesting that the latter is more reliable in determining antimicrobial activity. This article was authored by Singer Avalos Senthimalselvi, Panachimikumar, Allagymenavalin Lakshmi Prabha, and others.