 We will here present a new satraumethic method to evaluate cell health and apoptosis based on the arterial and novel and rapid apoptosis assay based on thyroid status. We will in the following introduce the background for the assay and demonstrate how the assay is carried out. This is a very complex cell life phenomenon and is characterized by a number of different morphological and biochemical changes. These changes can be used to assay apoptosis. As a few examples, the externalization of phosphatidyl siren can be detected using presently labeled NXN5. Caspase activity can be measured by different assays such as Flickr assay. Another phenomenon of apoptosis is the extrusion of reduced glutathione from the cell which creates a more oxidized environment inside the cell early in apoptosis. Reduced glutathione is the three peptide. Glutathione exists in two forms, the reduced form here and the oxidized form here. The reduced form will scavenge the cells from free radicals and by doing so will become in the oxidized form. The oxidized form can be reduced back to the reduced form under energy consumption and in the presence of glutathione by reductase. We can measure the amount of free thiols, the thiol level inside the cell in an assay for apoptosis. These about 48 reacts will reduce glutathione but not the oxidized form forming a strongly fluorescent product. The reaction is stoichiometric and we can thus directly measure the level of reduced glutathione in each cell by quantifying the presence of the cell. When running the analysis we will strain our sample with a mixture of VitaBrite 48 and Propetium iodide. Propetium iodide is included in the mixture to be able to distinguish non-viable cells of non-viable cells whether they die by necrosis or apoptosis or have a low level of reduced thiols. The sample can be either a control or a sample induced to undergo apoptosis. When we add up the stain we are ready to analyze the sample without any incubation or washing. So the sample is just directly analyzed either by flow cytometry or as in the article using image cytometry. We will get histograms of the sample above the control and below the apoptotic sample. The control of a higher general fluorescence intensity of the VitaBrite 48 staining while the apoptotic cells have a subpopulation with a low level of reduced thiols and hence a low staining with VitaBrite 48. We also see that in the apoptotic samples there are more non-viable cells which has to be gated out before the analysis. To quantify the level of apoptosis we can insert a marker which gives us a number on the cells which has a low level of reduced thiols and hence our apoptotic. The image shows jerker cells stained with anxin-5 in red, phytox-green in green and VitaBrite 48 in blue. The cells have been induced to undergo apoptosis using chemtuticin. Only a non-apoptotic cell that is not stained with the anxin-5, myra or phytox-green exhibit high VitaBrite fluorescence intensity.