 This study investigates the impact of ultrasonic energy and zinc oxide nanoparticles, ZNONPs, coating on thermophysiological properties of woven fabrics, including heat and mass transfer, using a lambita and moisture management tester. Thermophysiological parameters were applied to evaluate physical and overall comfort properties, thermal resistance, thermal diffusivity, heat flow, wetting time, and accumulative one-way transport index. The results show that ultrasonic energy affects surface roughness and topography of cotton and polyester fabrics, while ZNONPs coating impacts, textile substrates, surface morphology, and purity of crystal phase, as analyzed through scanning electron microscopy, energy dispersive x-ray, x-ray diffractometry, and inductively coupled plasma atomic emission spectroscopy. This article was authored by Muhammad Tayyab Nomen, Mikhail Petru, Petr Lauda, and others. We are article.tv, links in the description below.