 The results of this study suggest that frontline healthcare workers in Nepal have a satisfactory level of knowledge and practice regarding donning and doffing of personal protective equipment, PPE. However, there are some areas where improvement is needed. The study found that the profession of the participants, the provision of PPE at workplace, and the availability of designated spaces for donning and doffing of PPE were all significantly associated with satisfactory knowledge and practice scores. Additionally, the study also revealed that the sequence of donning and doffing of PPE was not known by most of the participants. Therefore, it is important to provide adequate training to frontline healthcare workers in order to improve their knowledge and practice regarding donning and doffing of PPE. This article was authored by Sagar Pandey, Sujan Pautil, Ashok Gher, and others.