 Now we want to test for reducing sugars and benediction reagent is the indicator that will determine if reducing sugars are present. Benediction reagent turns from a blue to another color such as yellow or red or orange or green if reducing sugars are present. So to do this we're going to put two dropper foils of water into one test tube and I have this labeled H2O. Then we're going to put two dropper foils of glucose solution into the other test tube. I have this one labeled G. For our next step we will add our benediction reagent to each test tube. So we'll go with 1, 2, 3, 4, 5, 6, 7 drops and 1, 2, 3, 4, 5, 6, 7 drops. You can see they're both a nice aqua shade of blue. The next step is to put each test tube into a boiling water bath. So the test tube with water is on the right and the test tube with the glucose solution is on the left. And we're looking for a color change, a change different from a color we presently have. And if you can see we're starting to get yellow forming in the test tube that contains glucose. So this is indicating the presence of a reducing sugar and glucose is reducing sugar. The water test tube has no color change. So the benediction reagent test for the presence of reducing sugars.