 The study aimed to identify new biomarkers for predicting bull fertility by analyzing the methylation levels of CPG sites in sperm-nuclear DNA using human methylation microarray and whole genome by sulfite sequence data. The results showed that 143 CPG sites were significantly differentially methylated, and 3 DMRs were selected through multiple regression analysis to predict bull fertility. This article was authored by Kumiko Takeda, Eji Kobayashi, Kazuko Ogata, and others.