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Published on Aug 2, 2012
Honors College student Pooja Patel -- "Investigation of Threshold Energy Absorption for HPPH-mediated PDT in Pancreatic Cancer Cells" -- Our research group has been working in collaboration with physicians at Mayo Clinic to develop a method of drug and light dosimetry for photodynamic therapy (PDT). We are studying Panc-1 pancreatic cancer cells with the photosensitizer 2-[1-Hexyloxyethyl]-2-devinyl Pyrophenophorbide-a (HPPH) and 670-nm light. The goal of my project is to determine whether there is a threshold of absorbed light for irreversible destruction of photosensitized Panc-1 pancreatic cancer cells. The cells are loaded with 2 mg/kg of HPPH and 670-nm light is applied at 0.5, 1.0, 1.5 and 2.0 J/cm2. Clonal assays are used to determine cell viability, accounting for cell death by both necrosis and apoptosis. After plotting the light absorption against cell viability, a threshold may be determined. This project will introduce a new set of data, which will be beneficial for the field of pancreatic cancer and Photodynamic Therapy.