 The present study has developed a novel biocompatible porous microneedle capable of rapid sampling, sensing, and glucose analysis in interstitial fluid, ISF. This microneedle contains glucose oxidase, GOX, and horseradish peroxidase, HRP, and a colorimetric sensing layer containing 3, 3-foot, 5, 5-tetramethylbenzadine, TMB. When the microneedle penetrates rat skin, it triggers the production of hydrogen peroxide, H202, from glucose. This reaction is then detected by the HRP and TMB combination, resulting in a visible color change. A smartphone analysis of the image quickly quantifies glucose levels in the 50-400 mg slash dl range. This microneedle-based sensing technique with minimally invasive sampling has great potential to improve patient compliance and detection efficiency. This article was authored by Qingya Zhang, Mingxin Su, Wei Luan Hu, and others. We are article.tv, links in the description below.