 Longitudinal analyses are needed to show how the immune response to SARS-CoV-2 infection changes over time. Here, the authors use multiple strategies to profile the change in immune cell responses from patients with convalescent COVID-19 over the course of five months, showing that although neutralizing antibody responses drop off after four months, B-cell immune responses strengthen. This article was authored by Adam K. Wheatley, Jennifer A. Juno, Jing J. Wang, and others.