 This study investigates the antimicrobial properties of efficiently synthesized biocompatible fluoroglysenol succinic acid, PGSA, dendrimer with anionic surfaces against gram-positive and gram-negative bacteria, as well as a fungal-human pathogen. Fluorescence and UV absorbance spectroscopy techniques were used to monitor the release of intracellular materials from the pathogens owing to anionic dendrimers. Molecular modeling studies revealed the exact binding sites of this dendrimer on these pathogens, motivating its use as a new antimicrobial agent. This article was authored by Murugasan Suresh Kumar, Subramani Karthikeyan, Shandra Segaran Ramprasad, and others.