 Human brain organoids are three-dimensional structures derived from pluripotent stem cells that mimic the structure and function of the developing human brain. They have been widely used as a tool for studying neurodevelopment and disease modeling. This paper provides a comprehensive review of the literature on human brain organoids, covering the various methods used to generate them, the cellular composition of the organoids, and the techniques used to assess their functionality. It also discusses the advantages and disadvantages of each method. Allowing researchers to make informed decisions when choosing a particular model. This article was authored by Lance Mulder, Jossé Depler, Auditia Shredor, and others.