 The abstract requirements for human mesenchymal stem cell-derived small extracellular vesicles, HMSC SEVs, are the first set of guidelines on HMSC SEVs in China. The guidelines were published by the Chinese Research Hospital Association on December 28, 2021 and became effective on January 1, 2022. These standards specify the technical requirements, testing methods, regulations, packaging, instructions for use, labeling, storage, transportation, and waste handling for HMSC SEVs. The goal of this guideline is to promote consensus on the research and application of HMSC SEVs and speed up their international standardization. This article was authored by Qin Li, Bo Li, Teng Yi, and others.