 It has been proposed that bacterial membrane proteins may be produced via transursion or concurrent transcription, translation and membrane insertion from membrane-associated genes. Provide evidence supporting that Vibrio parahemolyticus uses transursion to assemble the transmembrane complex type 3 secretion system used to inject virulence factors into host cells. This article was authored by Karin Gautam-Kaval, Sunita Chamalapati, Saadiz Eagle and others.