 The study identified 1575 no-murners and 68 novel-murners in the testus tissue of yearly goose and found that 71 differentially expressed murners and 660 differentially expressed genes were involved in sperm metabolism pathways. The results suggest that the sperm motility of yearly goose may be regulated by different murners, and target genes are significantly enriched in pathways related to sperm metabolism, indicating that murners affect the sperm motility of yearly goose by regulating the metabolic process of sperm and the expression of related genes. This article was authored by Yinping Wu, Li Huajong, Haiying Li, and others.