 This study investigated the effects of three different mixtures of N2, fixing plant growth promoting rhizobacteria on lettuce growth, yield, element content, and nitrate accumulation, as well as the reduction of nitrogen fertilization. The results showed that all treatments increased lettuce yield and growth, while inoculation with PGPR mixtures decreased heavy metal accumulation and increased nutrient uptake. The study found that using 25% less nitrogen fertilizer with a specific PGPR mixture, M3, can supply the same income as using the full dose of nitrogen fertilizer, making it an environmentally friendly option for crop production. This article was authored by Haluk, Keller, K-Mac, Adam Oxoy, and Rejep Kotin.