 This study found that healthcare professionals' unwillingness to prescribe pre-exposure prophylaxis, PREP, was associated with several factors, including non-prescription of HIV self-tests for key populations, lack of post-exposure prophylaxis, location of the specialized care services, SCS, in the state capital, and SCSs without PREP offer. Additionally, professionals who have not reported the need to conduct training and courses, or training with more experienced professionals, were also associated with a lower proportion unwillingness to prescribe PREP. These findings suggest that healthcare professionals' contextual, organizational, and training factors can contribute to PREP indication. To address this issue, it is important to expand ongoing training in combined HIV prevention among healthcare professionals and increase the availability of PREP in health services. This article was authored by Julianna de Souza-Lamanica, Leo Magno, Julia Elin Jesus de Silva Santos, and others.