 Nanoscale enzymes, known as nanosimes, have emerged as a promising alternative to traditional enzymes in biosensor applications due to their unique properties, such as high stability, robustness, and surface tunability. These features enable the development of highly sensitive and selective biosensors, which can detect trace amounts of analytes at the point of care. Recent research has focused on the integration of nanosimes into various sensing strategies, such as colorimetry, fluorescence, electrochemistry, and surface-enhanced Raman spectroscopy, SCRS. Despite the numerous advantages, challenges remain, such as stability and specificity, which must be addressed for widespread adoption. Future developments in this area promise to revolutionize healthcare by providing accurate, cost-effective, and portable diagnostic tools. This article was authored by Chitrapadmikumri Kharup and Minha Juddin Ahmed.