A Calcium-Dependent Immunocapture Strategy for Enhanced-Throughput SPR





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Published on Jan 17, 2013

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The applicability and throughput capacity of SPR-based assays are often limited by the time and expense involved in generating high-quality ligands and by difficulties in ensuring their integrity over multiple regeneration cycles. In this webinar, Dr John Kulman describes the development of a method to overcome these limitations utilizing reversible on-chip ligand capture from the complex medium of transiently transfected mammalian cells. Kulman and colleagues demonstrated the utility of this method in an SPR imaging array format using the ProteOn™ XPR36 system to determine the kinetic and thermodynamic parameters in antibody-antigen interactions, with dramatically reduced time and cost compared with traditional approaches.

John Kulman, PhD
Puget Sound Blood Center, USA


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