 Candida species are fungal pathogens that can cause both superficial and systemic infections in humans. They develop pathogenicity and multi-drug resistance traits, making it difficult to treat infections. One of these traits is the ability to form biofilms, which protects them from external factors such as the host's immune system and antifungal drugs. This review will discuss the differences between the four main species of Candida, including their ability to produce extracellular polymeric substances, EPS, dimorphism, and carbon source availability. It will also explore how the transcriptional control of processes like adhesion, biofilm formation, filamentation, and EPS production varies among the species. Finally, it will examine the implications of these differences on antifungal resistance and the potential strategies to limit biofilm formation or eradicate mature biofilms. This article was authored by Mafalda Cavalhero, Mafalda Cavalhero, Miguel Cacher Teixeira, and others.