 The study aimed to investigate the influence of HSA underscore CIRC underscore 0056558MIR-1290CDK6 axis on ankylosing spondylitis, AS, by analyzing differentially expressed genes and proteins, conducting QRTPCR, Western blotting, CCK8 assay, flow cytometry, bioinformatic analysis, and dual-leuciferase reporter assay. The findings revealed that HSA underscore CIRC underscore 0056558 and CDK6 suppress cell proliferation and differentiation while enhanced cell apoptosis by competitive binding to MIR-1290 in AS, which might be possibly achieved by PI3K, AKT, NFB pathway. This article was authored by Shia Li, Wenjing Du, Shim Li, and others.