 Hello, my name is Andrea Sabido and I'm a PhD student from the Institute of Biotechnology at the Universidad Nacional Autónoma de México. Hello, my name is Juan Carlos Igala from Universidad Autónoma Metropolitana Unidad Coagimalma. Through this video, we will show you the highlights of the recently accepted paper entitled Physiological and Transcriptional Characterization of Equalized Trains lacking interconversion of phosphonal pyruvate and pyruvate when glucose and acetate are coutilized. In this work, we describe features of the physiology of equalized trains, the void of the major glucose transport system in bacteria, the PTA system, during simultaneous utilization of glucose and acetate. We also provide information regarding their metabolic plasticity when carbon conversion between phosphonal pyruvate and pyruvate is blocked. And finally, we evaluated the impact of these generic modifications on phosphonal pyruvate availability by overproducing aromatic compounds on an engineered trains. In our group, we're interested in E. coli metabolism to develop metabolic engineering strategies to produce valuable compounds. For this reason, two E. coli mutants, the void of PTAs, were generated, the PV11 and PV12 strains. Both strains can in theory double PEPA bilability, which is one of the precursors of the aromatic compounds. However, due to the elimination of the PTA system, glucose transport and growth rates in the PV11 strain decreased significantly on glucose. To overcome this limitation, an adaptive evolution process on PV11 was performed and the PV12 strain was obtained, which partially recovered its growth rate on glucose compared with its parental strain. Due to the elimination of the 2A glucose component from the PTA system, these strains can consume simultaneously secondary carbon sources in the presence of glucose. Taking into account this capacity, we additionally blocked the PK, PKF and PPS genes and described the effects of these knockout mutations on the physiology of E. coli PTS minus strains during the utilization of glucose and acetate. In response to these knockout mutations, growth and specific consumption rates decreased significantly in the PV11 derivative compared with its parental strain. In addition, we show that genes involving transport and consumption of acetate, as well as some like redic genes, are down-regulated when there is no intervention of PEP and PIR. The same genetic modifications in the evolved PV12 derivative did not substantially affect these parameters during utilization of glucose and acetate. In fact, transcription of most genes did not change and only few were expressed. In this manuscript, we also demonstrated that by performing such mutations, phosphonal pyrovet derived from glucose catabolism can be directed towards the aromatic compounds biosynthetic pathway. The engineered strain derived from PV12, PK minus, PKF minus, PPS minus, enhance the final tidal and yield of aromatic compounds when co-utilizing glucose and acetate. Thanks for watching this video. If you have any questions, please contact us. Bye.