 FGF-19 plays an important role in cartilage development and physiology, and it has been identified as a potential therapeutic target for skeletal metabolic diseases. It has also been shown to stimulate mitochondrial biogenesis and fusion in chondrocytes, but the underlying mechanisms are not fully understood. This study investigates how FGF-19 affects mitochondrial biogenesis and fusion in chondrocytes, and reveals that this process is mediated by the binding of FGF-19 to its receptor, followed by activation of the AMPK-PGC-1-ALPHAT-SIRT-1 pathway. Furthermore, inhibiting the P38MAPK signaling pathway reduces the effects of FGF-19 on mitochondrial biogenesis and fusion, suggesting that this pathway may be involved in regulating the effects of FGF-19 on chondrocyte metabolism.