 Spiders are the most successful venomous animals and abundant terrestrial predators due to their ingenious use of silk and complex venoms that subjugate prey rapidly. Their venoms, dominated by disulfide-rich peptides, have high affinity and specificity for ion channels and receptors, making them a valuable resource for drug discovery against various pathophysiological conditions such as cardiovascular disorders, chronic pain, inflammation, and erectile dysfunction. This article was authored by Glenn F. King, Volker Herzig, Lachlan D. Rash, and others.