 This study aimed to utilize microbial proteins in wet processing of wool, specifically bacillus like aniformis ALW1 enzyme, which produced 52.1 UML enzyme activity and was thermophilic with optimum activity at pH 9. The effects of different treatment conditions on physical and chemical characteristics of wool were studied, including felting shrinkage, diability, amino acid analysis, alkali solubility, freotransform infrared spectroscopy, whiteness index, and tensile properties using scanning electron microscopy. The results showed that the modification of wool fibers with the produced enzyme highly improved their felting resistance and diability with acid dye without adverse effects on its inherent properties. This article was authored by Sheyma Agusmael, Mawa Abu Taleb, Mohammed A. Emron and others.