 gelatin hydrogels are currently being used to support three-dimensional, 3D, skin and vitro models. However, these materials lack the necessary mechanical properties and undergo rapid degradation, making them unsuitable for 3D in vitro cell culture. To overcome this issue, we investigated the potential of gelatin hydrogel with different concentrations cross-linked with genipin to promote human epidermal keratinocyte and human dermal fibroblast culture to develop a 3D in vitro skin model. We found that the cross-linked scaffolds had better physical properties, such as porosity and hydrophilicity, and genipin was found to enhance the physical properties. Additionally, no significant changes were observed in either formulation of cl underscore gl5 percent and cl underscore gl8 percent. The biocompatibility assay results indicated that all groups promoted cell attachment, cell viability, and cell migration, except for the cl underscore gl10 percent group. The cl underscore gl5 percent and cl underscore gl8 percent groups were selected to develop a bilayer 3D in vitro skin model. Amen. This article was authored by Safatari, Safatari, Manaira Maroff, and others. We are article.tv, links in the description below.