 This study examined the use of pleurotus florida to produce silver nanoparticles. The nanoparticles were characterized by UV visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. The x-ray diffraction studies and energy dispersive x-ray analysis indicated that the particles were crystalline in nature and had biomolecules on their surfaces. The nanoparticles showed excellent antimicrobial activity, making them suitable for use in various pharmaceutical applications. Furthermore, the synthetic process was environmentally friendly and easy to scale up, suggesting its potential for large-scale production. This article was authored by Ravishankarbot, Raghunandan Deshpande, Sharon Abbasava, V. Ganachari, and others.